| Item type |
学術雑誌論文 / Journal Article.(1) |
| 公開日 |
2020-08-04 |
| 書誌情報 |
en : Scientific Reports
巻 10,
発行日 2020
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| タイトル |
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タイトル |
Three-dimensional real time imaging of amyloid β aggregation on living cells |
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言語 |
en |
| 言語 |
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言語 |
eng |
| 資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
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資源タイプ |
journal article |
| アクセス権 |
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アクセス権 |
open access |
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アクセス権URI |
http://purl.org/coar/access_right/c_abf2 |
| 著者 |
倉賀野, 正弘
YAMASHITA, Ryota
CHIKAI, Yusaku
KITAMURA, Ryota
徳樂, 清孝
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| 室蘭工業大学研究者データベースへのリンク |
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表示名 |
徳樂 清孝（TOKURAKU Kiyotaka） |
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URL |
http://rdsoran.muroran-it.ac.jp/html/100000137_ja.html |
| 抄録 |
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内容記述タイプ |
Abstract |
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内容記述 |
Alzheimer’s disease (AD) is a progressive disorder of the brain that gradually decreases thinking, memory, and language abilities. The aggregation process of amyloid β (Aβ) is a key step in the expression of its neurocytotoxicity and development of AD because Aβ aggregation and accumulation around neuronal cells induces cell death. However, the molecular mechanism underlying the neurocytotoxicity and cell death by Aβ aggregation has not been clearly elucidated. In this study, we successfully visualized real-time process of Aβ42 aggregation around living cells by applying our established QD imaging method. 3D observations using confocal laser microscopy revealed that Aβ42 preferentially started to aggregate at the region where membrane protrusions frequently formed. Furthermore, we found that inhibition of actin polymerization using cytochalasin D reduced aggregation of Aβ42 on the cell surface. These results indicate that actin polymerization-dependent cell motility is responsible for the promotion of Aβ42 aggregation at the cell periphery. 3D observation also revealed that the aggregates around the cell remained in that location even if cell death occurred, implying that amyloid plaques found in the AD brain grew from the debris of dead cells that accumulated Aβ42 aggregates. |
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言語 |
ja |
| 出版者 |
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出版者 |
Springer Nature |
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言語 |
en |
| 出版者版へのリンク |
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表示名 |
10.1038/s41598-020-66129-z |
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URL |
https://doi.org/10.1038/s41598-020-66129-z |
| DOI |
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関連タイプ |
isIdenticalTo |
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識別子タイプ |
DOI |
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関連識別子 |
10.1038/s41598-020-66129-z |
| 日本十進分類法 |
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主題Scheme |
NDC |
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主題 |
499 |
| 権利 |
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言語 |
ja |
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権利情報 |
©NGO et.al. 2020 This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
| 著者版フラグ |
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出版タイプ |
VoR |
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出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| フォーマット |
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内容記述タイプ |
Other |
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内容記述 |
application/pdf |
SREP_10_9742 (8.6 MB)
