| Item type |
学術雑誌論文 / Journal Article.(1) |
| 公開日 |
2021-03-09 |
| 書誌情報 |
en : PLOS ONE
巻 8,
号 8,
発行日 2013
|
| タイトル |
|
|
タイトル |
A Microliter-Scale High-throughput Screening System with Quantum-Dot Nanoprobes for Amyloid-β Aggregation Inhibitors |
|
言語 |
en |
| 言語 |
|
|
言語 |
eng |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| アクセス権 |
|
|
アクセス権 |
open access |
|
アクセス権URI |
http://purl.org/coar/access_right/c_abf2 |
| 著者 |
ISHIGAKI, Yukako
TANAKA, Hiroyuki
AKAMA, Hiroaki
OGARA, Toshiki
上井, 幸司
徳樂, 清孝
|
| 室蘭工業大学研究者データベースへのリンク |
|
|
表示名 |
上井 幸司（UWAI Koji） |
|
URL |
http://rdsoran.muroran-it.ac.jp/html/100000240_ja.html |
| 室蘭工業大学研究者データベースへのリンク |
|
|
表示名 |
徳樂 清孝（TOKURAKU Kiyotaka） |
|
URL |
http://rdsoran.muroran-it.ac.jp/html/100000137_ja.html |
| 抄録 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
The aggregation of amyloid β protein (Aβ) is a key step in the pathogenesis of Alzheimer’s disease (AD), and therefore inhibitory substances for Aβ aggregation may have preventive and/or therapeutic potential for AD. Here we report a novel microliter-scale high-throughput screening system for Aβ aggregation inhibitors based on fluorescence microscopy-imaging technology with quantum-dot Nanoprobes. This screening system could be analyzed with a 5-µl sample volume when a 1536-well plate was used, and the inhibitory activity could be estimated as half-maximal effective concentrations (EC50). We attempted to comprehensively screen Aβ aggregation inhibitors from 52 spices using this system to assess whether this novel screening system is actually useful for screening inhibitors. Screening results indicate that approximately 90% of the ethanolic extracts from the spices showed inhibitory activity for Aβ aggregation. Interestingly, spices belonging to the Lamiaceae, the mint family, showed significantly higher activity than the average of tested spices. Furthermore, we tried to isolate the main inhibitory compound from Satureja hortensis, summer savory, a member of the Lamiaceae, using this system, and revealed that the main active compound was rosmarinic acid. These results demonstrate that this novel microliter-scale high-throughput screening system could be applied to the actual screening of Aβ aggregation inhibitors. Since this system can analyze at a microscopic scale, it is likely that further minimization of the system would easily be possible such as protein microarray technology. |
|
言語 |
en |
| 出版者 |
|
|
出版者 |
PUBLIC LIBRARY SCIENCE |
|
言語 |
en |
| 出版者版へのリンク |
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|
表示名 |
10.1371/journal.pone.0072992 |
|
URL |
https://doi.org/10.1371/journal.pone.0072992 |
| DOI |
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関連タイプ |
isIdenticalTo |
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|
識別子タイプ |
DOI |
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|
関連識別子 |
10.1371/journal.pone.0072992 |
| 日本十進分類法 |
|
|
主題Scheme |
NDC |
|
主題 |
499 |
| ISSN |
|
|
収録物識別子タイプ |
EISSN |
|
収録物識別子 |
1932-6203 |
| 権利 |
|
|
言語 |
en |
|
権利情報 |
https://creativecommons.org/licenses/by/4.0/ |
| 著者版フラグ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| フォーマット |
|
|
内容記述タイプ |
Other |
|
内容記述 |
application/pdf |
PO_8_8_e72992 (2.0 MB)
