| Item type |
学術雑誌論文 / Journal Article.(1) |
| 公開日 |
2016-05-16 |
| 書誌情報 |
en : Green Chemistry
巻 17,
号 9,
p. 4560-4569,
発行日 2015-09
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| タイトル |
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|
タイトル |
Degradation and conversion of toxic compounds into useful bioplastics by Cupriavidus sp. CY-1: relative expression of the PhaC gene under phenol and nitrogen stress |
|
言語 |
en |
| 言語 |
|
|
言語 |
eng |
| 資源タイプ |
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|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
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資源タイプ |
journal article |
| アクセス権 |
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|
アクセス権 |
open access |
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アクセス権URI |
http://purl.org/coar/access_right/c_abf2 |
| 著者 |
MOTAKATLA, Venkateswer Reddy
矢島, 由佳
馬渡, 康輝
星野, 保
張, 傛喆
|
| 室蘭工業大学研究者データベースへのリンク |
|
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表示名 |
矢島 由佳（YAJIMA Yuka） |
|
URL |
http://rdsoran.muroran-it.ac.jp/html/100000041_ja.html |
| 室蘭工業大学研究者データベースへのリンク |
|
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表示名 |
馬渡 康輝（MAWATARI Yasuteru） |
|
URL |
http://rdsoran.muroran-it.ac.jp/html/100000132_ja.html |
| 室蘭工業大学研究者データベースへのリンク |
|
|
表示名 |
張 傛喆（CHANG Young-Cheol） |
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URL |
http://rdsoran.muroran-it.ac.jp/html/100000221_ja.html |
| 抄録 |
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内容記述タイプ |
Abstract |
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内容記述 |
In this study different types of toxic compounds, i.e., alkylphenols, mono and poly-aromatic hydrocarbons were converted into polyhydroxybutyrate (PHB) using the isolated bacteria Cupriavidus sp. CY-1. The influence of Tween-80 on the toxic compound degradation ability of CY-1 was analyzed using high pressure liquid chromatography. Among all the compounds, CY-1 showed the highest removal of naphthalene (100 ± 6%), followed by phenol (96 ± 7%), and the lowest removal of alkylphenols without Tween-80 addition. However, Tween-80 addition enhanced the degradation capacity of CY-1, and showed the highest removal of 4-tertiary-butylphenol (74 ± 5%), followed by phenol (69 ± 5%), 4-chlorophenol (59 ± 3%), 4-tertiary-octylphenol (53 ± 5%), and naphthalene (48 ± 5%). Further experiments were carried out for conversion of toxic compounds into PHB. CY-1 grown with phenol (48 ± 6%) and naphthalene (42 ± 4%) showed the highest PHB production. The functional groups, structure and thermal properties of the produced PHB were analyzed. In addition the expression of the PhaC gene was quantified at the transcriptional level through real time quantitative PCR. The results showed up-regulation of the PhaC gene in the presence of phenol, and up and down-regulations in the presence of nitrogen. The maximum PhaC transcript expression was 5.37 folds at 100 mg l−1 nitrogen concentration. |
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言語 |
en |
| 出版者 |
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出版者 |
Royal Society of Chemistry |
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言語 |
en |
| 出版者版へのリンク |
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表示名 |
10.1039/C5GC01156F |
|
URL |
https://doi.org/10.1039/C5GC01156F |
| DOI |
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|
関連タイプ |
isVersionOf |
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|
識別子タイプ |
DOI |
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関連識別子 |
10.1039/C5GC01156F |
| 日本十進分類法 |
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主題Scheme |
NDC |
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主題 |
579 |
| ISSN |
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収録物識別子タイプ |
PISSN |
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収録物識別子 |
1463-9262 |
| ISSN |
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収録物識別子タイプ |
EISSN |
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収録物識別子 |
1463-9270 |
| 書誌レコードID |
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収録物識別子タイプ |
NCID |
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収録物識別子 |
AA11328146 |
| 権利 |
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言語 |
en |
|
権利情報 |
© 2015 The Royal Society of Chemistry |
| 著者版フラグ |
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出版タイプ |
AM |
|
出版タイプResource |
http://purl.org/coar/version/c_ab4af688f83e57aa |
| フォーマット |
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内容記述タイプ |
Other |
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内容記述 |
application/pdf |
GChem_17_9_4560-4569.pdf (1.4 MB)
