| Item type |
学術雑誌論文 / Journal Article.(1) |
| 公開日 |
2017-12-05 |
| 書誌情報 |
en : RSC Advances
巻 7,
号 34,
p. 20716-20723,
発行日 2017-04-11
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| タイトル |
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|
タイトル |
Biodegradation of aliphatic and aromatic hydrocarbons using the filamentous fungus Penicillium sp. CHY-2 and characterization of its manganese peroxidase activity |
|
言語 |
en |
| 言語 |
|
|
言語 |
eng |
| 資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
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資源タイプ |
journal article |
| アクセス権 |
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アクセス権 |
open access |
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アクセス権URI |
http://purl.org/coar/access_right/c_abf2 |
| 著者 |
Govarthanan, Muthusamy
藤澤, 宗一郎
細貝, 俊貴
張, 傛喆
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| 室蘭工業大学研究者データベースへのリンク |
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表示名 |
張 傛喆（CHANG Young-Cheol） |
|
URL |
http://rdsoran.muroran-it.ac.jp/html/100000221_ja.html |
| 抄録 |
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内容記述タイプ |
Abstract |
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内容記述 |
The aim of this work was to study the potential of the non-lignolytic filamentous fungus Penicillium sp. CHY-2, isolated from Antarctic soil, for the biodegradation of eight different aliphatic and aromatic hydrocarbons such as octane, decane, dodecane, ethylbenzene, butylbenzene, naphthalene, acenaphthene, and benzo[a]pyrene. Among all the compounds, CHY-2 showed the highest level of degradation for decane (49.0%), followed by butylbenzene (42.0%) and dodecane (33.0%), and lower levels of degradation for naphthalene (15.0%), acenaphthene (10.0%), octane (8.0%), ethylbenzene (4.0%), and benzo[a]pyrene (2.0%) at 20 °C. The addition of carbon sources such as glucose (5 g L−1) and Tween-80 (5 g L−1) enhanced decane degradation by about 1.8-fold and 1.61-fold respectively at 20 °C. The metabolites produced during the degradation of decane were identified by gas chromatography-mass spectrometry (GC-MS). Furthermore, the enzyme manganese peroxidase (MnP) from CHY-2 was purified. MnP was found to consist of monomers with a molecular mass of 36 kDa. The purified MnP had an optimum pH of 5.0 and temperature of 30 °C. The Km and Vmax values of MnP towards Mn2+ were 1.31 μM and 185.19 μM min−1 respectively. These results indicated that the strain CHY-2 can be used for the degradation of hydrocarbons and could have promising applications in treatment of hydrocarbon contaminated sites. |
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言語 |
en |
| 出版者 |
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出版者 |
The Royal Society of Chemistry |
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言語 |
en |
| 出版者版へのリンク |
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表示名 |
10.1039/C6RA28687A |
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URL |
https://dx.doi.org/10.1039/C6RA28687A |
| DOI |
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関連タイプ |
isIdenticalTo |
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識別子タイプ |
DOI |
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関連識別子 |
10.1039/C6RA28687A |
| 日本十進分類法 |
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主題Scheme |
NDC |
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主題 |
465 |
| 権利 |
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|
言語 |
en |
|
権利情報 |
This article is licensed under a Creative Commons Attribution 3.0 Unported Licence |
| 権利 |
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|
言語 |
en |
|
権利情報 |
© The Royal Society of Chemistry 2017 |
| 著者版フラグ |
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出版タイプ |
VoR |
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出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| フォーマット |
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内容記述タイプ |
Other |
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内容記述 |
appliction/PDF |
RSCAdv_7_20716-20723 (1.0 MB)
