Biodegradation of aliphatic and aromatic hydrocarbons using the filamentous fungus Penicillium sp. CHY-2 and characterization of its manganese peroxidase activity
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The aim of this work was to study the potential of the non-lignolytic filamentous fungus Penicillium sp. CHY-2, isolated from Antarctic soil, for the biodegradation of eight different aliphatic and aromatic hydrocarbons such as octane, decane, dodecane, ethylbenzene, butylbenzene, naphthalene, acenaphthene, and benzo[a]pyrene. Among all the compounds, CHY-2 showed the highest level of degradation for decane (49.0%), followed by butylbenzene (42.0%) and dodecane (33.0%), and lower levels of degradation for naphthalene (15.0%), acenaphthene (10.0%), octane (8.0%), ethylbenzene (4.0%), and benzo[a]pyrene (2.0%) at 20 °C. The addition of carbon sources such as glucose (5 g L−1) and Tween-80 (5 g L−1) enhanced decane degradation by about 1.8-fold and 1.61-fold respectively at 20 °C. The metabolites produced during the degradation of decane were identified by gas chromatography-mass spectrometry (GC-MS). Furthermore, the enzyme manganese peroxidase (MnP) from CHY-2 was purified. MnP was found to consist of monomers with a molecular mass of 36 kDa. The purified MnP had an optimum pH of 5.0 and temperature of 30 °C. The Km and Vmax values of MnP towards Mn2+ were 1.31 μM and 185.19 μM min−1 respectively. These results indicated that the strain CHY-2 can be used for the degradation of hydrocarbons and could have promising applications in treatment of hydrocarbon contaminated sites.
Biodegradation of aliphatic and aromatic hydrocarbons using the filamentous fungus Penicillium sp. CHY-2 and characterization of its manganese peroxidase activity
RSCAdv_7_20716-20723
(1.03MB)
