{"created":"2023-06-19T10:30:06.478505+00:00","id":10278,"links":{},"metadata":{"_buckets":{"deposit":"43014ab8-0738-4d94-9a3c-3b685e6bedbd"},"_deposit":{"created_by":18,"id":"10278","owners":[18],"pid":{"revision_id":0,"type":"depid","value":"10278"},"status":"published"},"_oai":{"id":"oai:muroran-it.repo.nii.ac.jp:00010278","sets":["216:371","46"]},"author_link":["57779","46701","57780","57048","58532","59063","57783"],"item_79_biblio_info_10":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2020","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"6","bibliographicVolumeNumber":"21","bibliographic_titles":[{"bibliographic_title":"International Journal of Molecular Sciences","bibliographic_titleLang":"en"}]}]},"item_79_description_23":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_79_description_7":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Amyloidosis refers to aggregates of protein that accumulate and are deposited as amyloid fibrils into plaques. When these are detected in organs, they are the main hallmark of Alzheimer’s disease, Parkinson’s disease, and other related diseases. Recent medical advances have shown that many precursors and proteins can induce amyloidosis even though the mechanism of amyloid aggregation and the relationship of these proteins to amyloidosis remains mostly unclear. In this study, we report the real-time 3D-imaging and inhibition analysis of amyloid B (AB ), tau, and a-synuclein aggregation utilizing the a nity between quantum dots (QD) and amyloid aggregates. We successfully visualized these amyloid aggregations in real-time using fluorescence microscopy and confocal microscopy simply by adding commercially available QD. The observation by transmission electron microscopy (TEM) showed that QD particles bound to all amyloid fibrils. The 3D-imaging with QD revealed di erences between amyloid aggregates composed of di erent amyloid peptides that could not be detected by TEM.We were also able to quantify the inhibition activities of these proteins by rosmarinic acid, which has high activity for AB aggregation, from fluorescence micrographs as half-maximal e ective concentrations. These imaging techniques with QD serve as quick, easy, and powerful tools to understand amyloidosis and to discover drugs for therapies.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_79_link_17":{"attribute_name":"出版者版へのリンク","attribute_value_mlt":[{"subitem_link_text":"10.3390/ijms21061978","subitem_link_url":"https://doi.org/10.3390/ijms21061978"}]},"item_79_link_5":{"attribute_name":"室蘭工業大学研究者データベースへのリンク","attribute_value_mlt":[{"subitem_link_text":"徳樂 清孝（TOKURAKU Kiyotaka）","subitem_link_url":"http://rdsoran.muroran-it.ac.jp/html/100000137_ja.html"}]},"item_79_publisher_11":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"MDPI","subitem_publisher_language":"en"}]},"item_79_relation_18":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.3390/ijms21061978","subitem_relation_type_select":"DOI"}}]},"item_79_rights_19":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"©2020 by the authors; licensee MDPI AG, Basel, Switzerland. This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution License(http://creativecommons.org/licenses/by/4.0/)","subitem_rights_language":"en"}]},"item_79_subject_9":{"attribute_name":"日本十進分類法","attribute_value_mlt":[{"subitem_subject":"499","subitem_subject_scheme":"NDC"}]},"item_79_version_type_21":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorAffiliations":[{"affiliationNameIdentifiers":[],"affiliationNames":[{"affiliationName":""}]}],"creatorNames":[{"creatorName":"LIN, Xuguang","creatorNameLang":"en"},{"creatorName":"リン, シュウグアン","creatorNameLang":"ja"}],"familyNames":[{},{}],"givenNames":[{},{}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"GALAQIN, Nuomin","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"TAINAKA, Reina","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorAffiliations":[{"affiliationNameIdentifiers":[],"affiliationNames":[{"affiliationName":""}]}],"creatorNames":[{"creatorName":"SHIMAMORI, Keiya","creatorNameLang":"en"},{"creatorName":"島森, 圭弥","creatorNameLang":"ja"},{"creatorName":"シマモリ, ケイヤ","creatorNameLang":"ja-Kana"}],"familyNames":[{},{},{}],"givenNames":[{},{},{}],"nameIdentifiers":[{}]},{"creatorAffiliations":[{"affiliationNameIdentifiers":[{}],"affiliationNames":[{},{}]}],"creatorNames":[{"creatorName":"KURAGANO, Masahiro","creatorNameLang":"en"},{"creatorName":"倉賀野, 正弘","creatorNameLang":"ja"},{"creatorName":"クラガノ, マサヒロ","creatorNameLang":"ja-Kana"}],"familyNames":[{},{},{}],"givenNames":[{},{},{}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"NOGUCHI, Taro. Q. P","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorAffiliations":[{"affiliationNameIdentifiers":[{}],"affiliationNames":[{},{}]}],"creatorNames":[{"creatorName":"TOKURAKU, Kiyotaka","creatorNameLang":"en"},{"creatorName":"トクラク, キヨタカ","creatorNameLang":"ja-Kana"},{"creatorName":"徳樂, 清孝","creatorNameLang":"ja"}],"familyNames":[{},{},{}],"givenNames":[{},{},{}],"nameIdentifiers":[{},{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-07-09"}],"displaytype":"detail","filename":"IJMS_2020_21_1978.pdf","filesize":[{"value":"2.1 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"IJMS_2020_21_1978","objectType":"fulltext","url":"https://muroran-it.repo.nii.ac.jp/record/10278/files/IJMS_2020_21_1978.pdf"},"version_id":"fb66ccdd-080e-4a4e-89d9-aeb5202f1241"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"amyloidosis; amyloid β","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"tau","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"α-synuclein","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"amyloid aggregation inhibitor","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"quantum dot","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"4D-imaging","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Real-Time 3D Imaging and Inhibition Analysis of Various Amyloid Aggregations Using Quantum Dots","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Real-Time 3D Imaging and Inhibition Analysis of Various Amyloid Aggregations Using Quantum Dots","subitem_title_language":"en"}]},"item_type_id":"79","owner":"18","path":["46","371"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2020-07-09"},"publish_date":"2020-07-09","publish_status":"0","recid":"10278","relation_version_is_last":true,"title":["Real-Time 3D Imaging and Inhibition Analysis of Various Amyloid Aggregations Using Quantum Dots"],"weko_creator_id":"18","weko_shared_id":-1},"updated":"2023-12-26T07:32:04.127623+00:00"}