{"created":"2023-06-19T10:30:16.417106+00:00","id":10545,"links":{},"metadata":{"_buckets":{"deposit":"ccd5f6b8-ba7f-4806-a41e-958acc73b156"},"_deposit":{"created_by":18,"id":"10545","owners":[18],"pid":{"revision_id":0,"type":"depid","value":"10545"},"status":"published"},"_oai":{"id":"oai:muroran-it.repo.nii.ac.jp:00010545","sets":["216:312","46"]},"author_link":["58818","58817","20833","55087","58821","58820"],"item_79_biblio_info_10":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2021","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"11","bibliographicPageStart":"157","bibliographicVolumeNumber":"8","bibliographic_titles":[{"bibliographic_title":"BIOENGINEERING-BASEL","bibliographic_titleLang":"en"}]}]},"item_79_description_23":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_79_description_7":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Cheese whey (CW) can be an excellent carbon source for polyhydroxyalkanoates (PHA)-producing bacteria. Most studies have used CW, which contains high amounts of lactose, however, there are no reports using raw CW, which has a relatively low amount of lactose. Therefore, in the present study, PHA production was evaluated in a two-stage process using the CW that contains low amounts of lactose. In first stage, the carbon source existing in CW was converted into acetic acid using the bacteria, Acetobacter pasteurianus C1, which was isolated from food waste. In the second stage, acetic acid produced in the first stage was converted into PHA using the bacteria, Bacillus sp. CYR-1. Under the condition of without the pretreatment of CW, acetic acid produced from CW was diluted at different folds and used for the production of PHA. Strain CYR-1 incubated with 10-fold diluted CW containing 5.7 g/L of acetic acid showed the higher PHA production (240.6 mg/L), whereas strain CYR-1 incubated with four-fold diluted CW containing 12.3 g/L of acetic acid showed 126 mg/L of PHA. After removing the excess protein present in CW, PHA production was further enhanced by 3.26 times (411 mg/L) at a four-fold dilution containing 11.3 g/L of acetic acid. Based on Fourier transform infrared spectroscopy (FT-IR), and H-1 and C-13 nuclear magnetic resonance (NMR) analyses, it was confirmed that the PHA produced from the two-stage process is poly-beta-hydroxybutyrate (PHB). All bands appearing in the FT-IR spectrum and the chemical shifts of NMR nearly matched with those of standard PHB. Based on these studies, we concluded that a two-stage process using Acetobacter pasteurianus C1 and Bacillus sp. CYR-1 would be applicable for the production of PHB using CW containing a low amount of lactose.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_79_link_17":{"attribute_name":"出版者版へのリンク","attribute_value_mlt":[{"subitem_link_text":"10.3390/bioengineering8110157","subitem_link_url":"https://doi.org/10.3390/bioengineering8110157"}]},"item_79_link_5":{"attribute_name":"室蘭工業大学研究者データベースへのリンク","attribute_value_mlt":[{"subitem_link_text":"張 傛喆（CHANG Young-Cheol）","subitem_link_url":"https://rdsoran.muroran-it.ac.jp/html/100000221_ja.html"}]},"item_79_publisher_11":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"MDPI","subitem_publisher_language":"en"}]},"item_79_relation_18":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.3390/bioengineering8110157","subitem_relation_type_select":"DOI"}}]},"item_79_rights_19":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"https://creativecommons.org/licenses/by/4.0/","subitem_rights_language":"en"}]},"item_79_subject_9":{"attribute_name":"日本十進分類法","attribute_value_mlt":[{"subitem_subject":"464","subitem_subject_scheme":"NDC"}]},"item_79_version_type_21":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorAffiliations":[{"affiliationNameIdentifiers":[{}],"affiliationNames":[{},{}]}],"creatorNames":[{"creatorName":"CHANG,  Young-Cheol","creatorNameLang":"en"},{"creatorName":"チャン, ヨンチョル","creatorNameLang":"ja-Kana"},{"creatorName":"張, 傛喆","creatorNameLang":"ja"}],"familyNames":[{},{},{}],"givenNames":[{},{},{}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"Reddy, Motakatla Venkateswar","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Imura, Kazuma","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorAffiliations":[{"affiliationNameIdentifiers":[],"affiliationNames":[{"affiliationName":""}]}],"creatorNames":[{"creatorName":"ONODERA, Rui","creatorNameLang":"en"},{"creatorName":"小野寺, 瑠依","creatorNameLang":"ja"},{"creatorName":"オノデラ, ルイ","creatorNameLang":"ja-Kana"}],"familyNames":[{},{},{}],"givenNames":[{},{},{}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kamada, Natsumi","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Sano, Yuki","creatorNameLang":"en"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2022-03-24"}],"displaytype":"detail","filename":"Bioeng_8_11_157.pdf","filesize":[{"value":"5.2 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"Bioeng_8_11_157","objectType":"fulltext","url":"https://muroran-it.repo.nii.ac.jp/record/10545/files/Bioeng_8_11_157.pdf"},"version_id":"b83e9f40-7b27-4678-8f36-d5e4f768084b"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"cheese whey","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"acetic acid","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Acetobacter pasteurianus C1","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Bacillus sp. CYR-1","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"PHA","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"PHB","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Two-Stage Polyhydroxyalkanoates (PHA) Production from Cheese Whey Using Acetobacter pasteurianus C1 and Bacillus sp. CYR1","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Two-Stage Polyhydroxyalkanoates (PHA) Production from Cheese Whey Using Acetobacter pasteurianus C1 and Bacillus sp. CYR1","subitem_title_language":"en"}]},"item_type_id":"79","owner":"18","path":["46","312"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2022-03-24"},"publish_date":"2022-03-24","publish_status":"0","recid":"10545","relation_version_is_last":true,"title":["Two-Stage Polyhydroxyalkanoates (PHA) Production from Cheese Whey Using Acetobacter pasteurianus C1 and Bacillus sp. CYR1"],"weko_creator_id":"18","weko_shared_id":-1},"updated":"2024-02-05T07:07:04.688017+00:00"}