{"created":"2023-06-19T10:30:28.598175+00:00","id":10911,"links":{},"metadata":{"_buckets":{"deposit":"d008cc3a-ad45-4d1b-80ed-7bc99f930c9e"},"_deposit":{"created_by":18,"id":"10911","owners":[18],"pid":{"revision_id":0,"type":"depid","value":"10911"},"status":"published"},"_oai":{"id":"oai:muroran-it.repo.nii.ac.jp:00010911","sets":["216:371","216:380","46"]},"author_link":["46701","57048","41919"],"item_79_biblio_info_10":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2022","bibliographicIssueDateType":"Issued"},"bibliographicPageStart":"112449","bibliographicVolumeNumber":"214","bibliographic_titles":[{"bibliographic_title":"COLLOIDS AND SURFACES B-BIOINTERFACES","bibliographic_titleLang":"en"}]}]},"item_79_description_23":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_79_description_7":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Abnormal aggregation and accumulation of misfolded proteins are involved in the development of various forms of amyloidosis. Aggregates that accumulate in organs induce an inflammatory response and cytotoxicity, and lead to organ failure. Although protein accumulation around an affected area in the body is an important stage that is directly linked to the mechanism of pathogenesis, the kinetics of the accumulation of protein that precipitates while assembling is not well understood because 3D tracking of proteins in solution is difficult. Here, we analyzed the process of aggregation and accumulation of amyloid beta (A beta), which causes the development of Alzheimer's disease (AD), by real-time 3D imaging under physiological conditions using a quantum dot nanoprobe that we previously developed. 3D observations demonstrated that A beta aggregates with a diameter of several mu m emerged in phosphate-buffered saline, gathered in a spiral-like step, and exhibited a mesh-like structure. Additionally, we found that the amount and size of aggregates decreased dramatically in 40% glycerol solution, mimicking the viscosity of human blood. We confirmed that fibrils in 40% glycerol exhibited an extremely short and tangled morphology and formed dense aggregates. Furthermore, numerical calculations revealed that several decades are required to fully develop the settling velocity and diameter of A beta aggregates in physiological conditions. This time span is consistent with the actual symptom progression of AD.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_79_link_17":{"attribute_name":"出版者版へのリンク","attribute_value_mlt":[{"subitem_link_text":"10.1016/j.colsurfb.2022.112449","subitem_link_url":"https://doi.org/10.1016/j.colsurfb.2022.112449"}]},"item_79_link_5":{"attribute_name":"室蘭工業大学研究者データベースへのリンク","attribute_value_mlt":[{"subitem_link_text":"山中 真也（YAMANAKA Shinya）","subitem_link_url":"http://rdsoran.muroran-it.ac.jp/html/100000163_ja.html"},{"subitem_link_text":"徳樂 清孝（TOKURAKU Kiyotaka）","subitem_link_url":"http://rdsoran.muroran-it.ac.jp/html/100000137_ja.html"}]},"item_79_publisher_11":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"ELSEVIER","subitem_publisher_language":"en"}]},"item_79_relation_18":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1016/j.colsurfb.2022.112449","subitem_relation_type_select":"DOI"}}]},"item_79_relation_20":{"attribute_name":"参考URL","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_language":"ja","subitem_relation_name_text":"著者版PDFダウンロード"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://www.sciencedirect.com/science/article/am/pii/S0927776522001321","subitem_relation_type_select":"URI"}}]},"item_79_source_id_12":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0927-7765","subitem_source_identifier_type":"PISSN"}]},"item_79_subject_9":{"attribute_name":"日本十進分類法","attribute_value_mlt":[{"subitem_subject":"437","subitem_subject_scheme":"NDC"}]},"item_79_version_type_21":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifier":"10103","affiliationNameIdentifierScheme":"kakenhi"}],"affiliationNames":[{"affiliationName":"室蘭工業大学","affiliationNameLang":"ja"},{"affiliationName":"Muroran Institute of Technology","affiliationNameLang":"en"}]}],"creatorNames":[{"creatorName":"KURAGANO, Masahiro","creatorNameLang":"en"},{"creatorName":"倉賀野, 正弘","creatorNameLang":"ja"},{"creatorName":"クラガノ, マサヒロ","creatorNameLang":"ja-Kana"}],"familyNames":[{"familyName":"KURAGANO","familyNameLang":"en"},{"familyName":"倉賀野","familyNameLang":"ja"},{"familyName":"クラガノ","familyNameLang":"ja-Kana"}],"givenNames":[{"givenName":"Masahiro","givenNameLang":"en"},{"givenName":"正弘","givenNameLang":"ja"},{"givenName":"マサヒロ","givenNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"57048","nameIdentifierScheme":"WEKO"}]},{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifier":"10103","affiliationNameIdentifierScheme":"kakenhi"}],"affiliationNames":[{"affiliationName":"室蘭工業大学","affiliationNameLang":"ja"},{"affiliationName":"Muroran Institute of Technology","affiliationNameLang":"en"}]}],"creatorNames":[{"creatorName":"山中,  真也","creatorNameLang":"ja"},{"creatorName":"YAMANAKA, Shinya","creatorNameLang":"en"},{"creatorName":"ヤマナカ, シンヤ","creatorNameLang":"ja-Kana"}],"familyNames":[{"familyName":"山中","familyNameLang":"ja"},{"familyName":"YAMANAKA","familyNameLang":"en"},{"familyName":"ヤマナカ","familyNameLang":"ja-Kana"}],"givenNames":[{"givenName":" 真也","givenNameLang":"ja"},{"givenName":"Shinya","givenNameLang":"en"},{"givenName":"シンヤ","givenNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"41919","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"1000030596854","nameIdentifierScheme":"NRID","nameIdentifierURI":"https://nrid.nii.ac.jp/ja/nrid/1000030596854"}]},{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifier":"10103","affiliationNameIdentifierScheme":"kakenhi"}],"affiliationNames":[{"affiliationName":"室蘭工業大学","affiliationNameLang":"ja"},{"affiliationName":"Muroran Institute of Technology","affiliationNameLang":"en"}]}],"creatorNames":[{"creatorName":"TOKURAKU, Kiyotaka","creatorNameLang":"en"},{"creatorName":"トクラク, キヨタカ","creatorNameLang":"ja-Kana"},{"creatorName":"徳樂, 清孝","creatorNameLang":"ja"}],"familyNames":[{"familyName":"TOKURAKU","familyNameLang":"en"},{"familyName":"トクラク","familyNameLang":"ja-Kana"},{"familyName":"徳樂","familyNameLang":"ja"}],"givenNames":[{"givenName":"Kiyotaka","givenNameLang":"en"},{"givenName":"キヨタカ","givenNameLang":"ja-Kana"},{"givenName":"清孝","givenNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"46701","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"1000000332106","nameIdentifierScheme":"NRID","nameIdentifierURI":"https://nrid.nii.ac.jp/ja/nrid/1000000332106"}]}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Alzheimer's disease","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Amyloid beta","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Aggregation and accumulation","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Quantum dots","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Numerical calculations","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Kinetics of amyloid accumulation in physiological viscosity","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Kinetics of amyloid accumulation in physiological viscosity","subitem_title_language":"en"}]},"item_type_id":"79","owner":"18","path":["46","380","371"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2024-01-24"},"publish_date":"2024-01-24","publish_status":"0","recid":"10911","relation_version_is_last":true,"title":["Kinetics of amyloid accumulation in physiological viscosity"],"weko_creator_id":"18","weko_shared_id":-1},"updated":"2023-10-25T00:43:00.734184+00:00"}