{"created":"2023-10-19T05:48:58.658113+00:00","id":2000095,"links":{},"metadata":{"_buckets":{"deposit":"a6167119-fc3f-48d9-86c5-0b14a5cbc2b2"},"_deposit":{"created_by":20,"id":"2000095","owner":"20","owners":[20],"pid":{"revision_id":0,"type":"depid","value":"2000095"},"status":"published"},"_oai":{"id":"oai:muroran-it.repo.nii.ac.jp:02000095","sets":["216:474","46"]},"author_link":[],"item_79_biblio_info_10":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2023-02-05","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"6","bibliographicNumberOfPages":"9","bibliographicPageEnd":"1444","bibliographicPageStart":"1436","bibliographicVolumeNumber":"127","bibliographic_titles":[{"bibliographic_title":"The Journal of Physical Chemistry A","bibliographic_titleLang":"en"}]}]},"item_79_description_7":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Thioflavin T (ThT) is a typical fluorescent marker for detecting the formation of amyloid fibrils, because its fluorescence intensity increases by more than two orders of magnitude upon complexation with the fibrils. Strong electrostatic fields on protein surfaces are known to be a significant factor in chemical reactions and biological functions. Therefore, ThT bound to amyloid fibrils must experience strong electric fields. This study employed electroabsorption and Stark fluorescence spectroscopies to clarify the effects of external electric fields on the photophysics of ThT. The absorption spectrum shows two bands ascribed to locally excited (LE) and charge transfer (CT) states. Coupling between the LE and CT states is enhanced in the presence of an external electric field, resulting in fluorescence quenching. The electric field strength of the amyloid fibril surface was inferred from the fluorescence quenching efficiency of ThT.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_79_link_17":{"attribute_name":"出版者版へのリンク","attribute_value_mlt":[{"subitem_link_url":"https://doi.org/10.1021/acs.jpca.2c07794"}]},"item_79_publisher_11":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"American Chemical Society","subitem_publisher_language":"en"}]},"item_79_relation_18":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1021/acs.jpca.2c07794","subitem_relation_type_select":"DOI"}}]},"item_79_rights_19":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"© 2023 American Chemical Society","subitem_rights_language":"en"}]},"item_79_source_id_12":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1089-5639","subitem_source_identifier_type":"PISSN"}]},"item_79_version_type_21":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Islam, Ahatashamul","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Kikuchi, Yudai","creatorNameLang":"en"}]},{"creatorAffiliations":[{"affiliationNames":[{},{}]}],"creatorNames":[{"creatorName":"Iimori, Toshifumi","creatorNameLang":"en"},{"creatorName":"飯森, 俊文","creatorNameLang":"ja"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2024-02-05"}],"displaytype":"detail","filename":"Manuscript_thioflavinT-R1-3-A.pdf","filesize":[{"value":"943 KB"}],"format":"application/pdf","url":{"url":"https://muroran-it.repo.nii.ac.jp/record/2000095/files/Manuscript_thioflavinT-R1-3-A.pdf"},"version_id":"7afce1bc-33a1-4f1a-94c2-b474d00c7ad2"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Electroabsorption and Stark Fluorescence Spectroscopies of Thioflavin T","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Electroabsorption and Stark Fluorescence Spectroscopies of Thioflavin T","subitem_title_language":"en"}]},"item_type_id":"79","owner":"20","path":["46","474"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2024-02-05"},"publish_date":"2024-02-05","publish_status":"0","recid":"2000095","relation_version_is_last":true,"title":["Electroabsorption and Stark Fluorescence Spectroscopies of Thioflavin T"],"weko_creator_id":"20","weko_shared_id":-1},"updated":"2023-10-19T06:00:41.847830+00:00"}