| アイテムタイプ |
学術雑誌論文 / Journal Article.(1) |
| 公開日 |
2023-10-24 |
| 書誌情報 |
en : Life Science Alliance
巻 6,
号 1,
ページ数 18,
発行日 2023
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| タイトル |
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|
タイトル |
Actin-binding domain of Rng2 sparsely bound on F-actin strongly inhibits actin movement on myosin II |
|
言語 |
en |
| 言語 |
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|
言語 |
eng |
| 資源タイプ |
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|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
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資源タイプ |
journal article |
| アクセス権 |
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アクセス権 |
open access |
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アクセス権URI |
http://purl.org/coar/access_right/c_abf2 |
| 著者 |
Hayakawa, Yuuki
Takaine, Masak
Ngo, Kien Xuan
Imai, Taiga
Yamada, Masafumi D
Behjat, Arash Badami
Umeda, Kenichi
Hirose, Keiko
Yurtsever, Ayhan
Kodera, Noriyuki
徳楽, 清孝
Numata, Osamu
Fukuma, Takeshi
Ando, Toshio
Nakano, Kentaro
Uyeda, Taro QP
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| 抄録 |
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内容記述タイプ |
Abstract |
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内容記述 |
We report a case in which sub-stoichiometric binding of an actin-binding protein has profound structural and functional consequences, providing an insight into the fundamental properties of actin regulation. Rng2 is an IQGAP contained in contractile rings in the fission yeast Schizosaccharomyces pombe. Here, we used high-speed atomic force microscopy and electron microscopy and found that sub-stoichiometric binding of the calponin-homology actin-binding domain of Rng2 (Rng2CHD) induces global structural changes in skeletal muscle actin filaments, including shortening of the filament helical pitch. Sub-stoichiometric binding of Rng2CHD also reduced the affinity between actin filaments and muscle myosin II carrying ADP and strongly inhibited the motility of actin filaments on myosin II in vitro. On skeletal muscle myosin II–coated surfaces, Rng2CHD stopped the actin movements at a binding ratio of 11%. Rng2CHD also inhibited actin movements on myosin II of the amoeba Dictyostelium, but in this case, by detaching actin filaments from myosin II–coated surfaces. Thus, sparsely bound Rng2CHD induces apparently cooperative structural changes in actin filaments and inhibits force generation by actomyosin II. |
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言語 |
en |
| 出版者 |
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出版者 |
Life Science Alliance LLC |
|
言語 |
en |
| DOI |
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関連タイプ |
isIdenticalTo |
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識別子タイプ |
DOI |
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関連識別子 |
10.26508/lsa.202201469 |
| ISSN |
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収録物識別子タイプ |
EISSN |
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収録物識別子 |
2575-1077 |
| 権利 |
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|
権利情報 |
© 2022 Hayakawa et al. |
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言語 |
en |
| 著者版フラグ |
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出版タイプ |
VoR |
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出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
e202201469.full.pdf (3 MB)
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