@article{oai:muroran-it.repo.nii.ac.jp:00008239, author = {馬, 娟 and MA, Juang and 菊池, 愼太郎 and KIKUCHI, Shintaro}, journal = {室蘭工業大学研究報告. 理工編, Memoirs of the Muroran Institute of Technology. Science and engineering}, month = {Nov}, note = {application/pdf, An enzyme immunoassay using antiserum against fatty acid synthetase I(FAS-I; a malony1-CoAdependent,acy1 carrier protein (ACP)-requiring system) from an avirulent mycobacteria,Mycobacterium smegmatis,was examined for detection of cells of this genus. The antiserum was cross-reactive with virulent and avirulent species of mycobacteria such as M.tuberculosis,M.bovis BCG and M.phlei,respectively. Only a weak reactivity was,however,observed with Nocardia sp. possessing both FAS-I and FAS-II(fatty acid synthetase II ; an acety1-CoA-dependent,ACP-requiring system),and no cross-reactivities were found with bacteria like Eschelichia coli and Bacillus subtilis having only FAS-II. These results suggested that virulent and avirulent Mycobacterium sp. could be detected and identified specifically by the immunoassay method using the enzyme-labeled antiserum aganst FAS-I.}, pages = {1--7}, title = {酵素標識免疫法によるヒト型結核菌および近縁菌群の検出}, volume = {46}, year = {1996}, yomi = {キクチ, シンタロウ} }