{"created":"2023-06-19T10:29:40.577523+00:00","id":9641,"links":{},"metadata":{"_buckets":{"deposit":"848a64a9-80ed-47ad-a326-8dc257d8dd76"},"_deposit":{"created_by":18,"id":"9641","owners":[18],"pid":{"revision_id":0,"type":"depid","value":"9641"},"status":"published"},"_oai":{"id":"oai:muroran-it.repo.nii.ac.jp:00009641","sets":["216:311","216:312","216:372","46"]},"author_link":["55086","53486","20833","55087","56671","56124"],"item_79_biblio_info_10":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2017-06","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"105","bibliographicPageStart":"99","bibliographicVolumeNumber":"234","bibliographic_titles":[{"bibliographic_title":"Bioresource Technology","bibliographic_titleLang":"en"}]}]},"item_79_description_23":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"applicaton/pdf","subitem_description_type":"Other"}]},"item_79_description_7":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Synthetic wastewater (SW) at various carbon concentrations (5–60 g/l) were evaluated for polyhydroxyalkanoates (PHA) production using the bacteria Pseudomonas pseudoflava. Bacteria showed highest PHA production with 20 g/l (57 ± 5%), and highest carbon removal at 5 g/l (74 ± 6%) concentrations respectively. Structure, molecular weight, and thermal properties of the produced PHA were evaluated using various analytical techniques. Bacteria produced homo-polymer [poly-3-hydroxybutyrate (P3HB)] when only acetate was used as carbon source; and it produced co-polymer [poly-(3-hydroxybutyrate-co-3-hydroxyvalerate) P(3HB-co-3HV)] by addition of co-substrate propionate. PHA synthase, the enzyme which produce PHA was extracted from two bacterial strains i.e., P. pseudoflava and P. palleronii and its molecular weight was analysed using SDS-PAGE. Protein concentration, and PHA synthase enzyme activity of P. pseudoflava and P. palleronii was carried out using spectrophotometer. Results denoted that P. pseudoflava can be used for degradation of organic carbon persistent in wastewaters and their subsequent conversion into PHA.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_79_link_17":{"attribute_name":"出版者版へのリンク","attribute_value_mlt":[{"subitem_link_text":"10.1016/j.biortech.2017.03.008","subitem_link_url":"https://doi.org/10.1016/j.biortech.2017.03.008"}]},"item_79_link_5":{"attribute_name":"室蘭工業大学研究者データベースへのリンク","attribute_value_mlt":[{"subitem_link_text":"馬渡 康輝（MAWATARI Yasuteru）","subitem_link_url":"http://rdsoran.muroran-it.ac.jp/html/100000132_ja.html"},{"subitem_link_text":"矢島 由佳（YAJIMA Yuka）","subitem_link_url":"http://rdsoran.muroran-it.ac.jp/html/100000041_ja.html"},{"subitem_link_text":"張 傛喆（CHANG Young-Cheol）","subitem_link_url":"http://rdsoran.muroran-it.ac.jp/html/100000221_ja.html"}]},"item_79_publisher_11":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Elsevier","subitem_publisher_language":"en"}]},"item_79_relation_18":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1016/j.biortech.2017.03.008","subitem_relation_type_select":"DOI"}}]},"item_79_rights_19":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"© 2016. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/","subitem_rights_language":"en"}]},"item_79_source_id_12":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"09608524","subitem_source_identifier_type":"PISSN"}]},"item_79_subject_9":{"attribute_name":"日本十進分類法","attribute_value_mlt":[{"subitem_subject":"430","subitem_subject_scheme":"NDC"}]},"item_79_version_type_21":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorAffiliations":[{"affiliationNameIdentifiers":[],"affiliationNames":[{"affiliationName":""}]}],"creatorNames":[{"creatorName":"REDDY, M. Venkateswar","creatorNameLang":"en"}],"familyNames":[{}],"givenNames":[{}],"nameIdentifiers":[{}]},{"creatorAffiliations":[{"affiliationNameIdentifiers":[{}],"affiliationNames":[{},{}]}],"creatorNames":[{"creatorName":"MAWATARI, Yasuteru","creatorNameLang":"en"},{"creatorName":"馬渡, 康輝","creatorNameLang":"ja"},{"creatorName":"マワタリ, ヤステル","creatorNameLang":"ja-Kana"}],"familyNames":[{},{},{}],"givenNames":[{},{},{}],"nameIdentifiers":[{},{}]},{"creatorAffiliations":[{"affiliationNameIdentifiers":[],"affiliationNames":[{"affiliationName":""}]}],"creatorNames":[{"creatorName":"ONODERA, Rui","creatorNameLang":"en"},{"creatorName":"小野寺, 瑠依","creatorNameLang":"ja"},{"creatorName":"オノデラ, ルイ","creatorNameLang":"ja-Kana"}],"familyNames":[{},{},{}],"givenNames":[{},{},{}],"nameIdentifiers":[{}]},{"creatorAffiliations":[{"affiliationNameIdentifiers":[],"affiliationNames":[{"affiliationName":""}]}],"creatorNames":[{"creatorName":"NAKAMURA, Yuki","creatorNameLang":"en"},{"creatorName":"中村, 友綺","creatorNameLang":"ja"},{"creatorName":"ナカムラ, ユウキ","creatorNameLang":"ja-Kana"}],"familyNames":[{},{},{}],"givenNames":[{},{},{}],"nameIdentifiers":[{}]},{"creatorAffiliations":[{"affiliationNameIdentifiers":[{}],"affiliationNames":[{},{}]}],"creatorNames":[{"creatorName":"矢島, 由佳","creatorNameLang":"ja"},{"creatorName":"YAJIMA, Yuka","creatorNameLang":"en"},{"creatorName":"ヤジマ, ユカ","creatorNameLang":"ja-Kana"}],"familyNames":[{},{},{}],"givenNames":[{},{},{}],"nameIdentifiers":[{},{}]},{"creatorAffiliations":[{"affiliationNameIdentifiers":[{}],"affiliationNames":[{},{}]}],"creatorNames":[{"creatorName":"CHANG,  Young-Cheol","creatorNameLang":"en"},{"creatorName":"チャン, ヨンチョル","creatorNameLang":"ja-Kana"},{"creatorName":"張, 傛喆","creatorNameLang":"ja"}],"familyNames":[{},{},{}],"givenNames":[{},{},{}],"nameIdentifiers":[{},{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2019-06-01"}],"displaytype":"detail","filename":"BT_234_99_105.pdf","filesize":[{"value":"921.1 kB"}],"format":"application/pdf","licensetype":"license_5","mimetype":"application/pdf","url":{"label":"BT_234_99_105","objectType":"fulltext","url":"https://muroran-it.repo.nii.ac.jp/record/9641/files/BT_234_99_105.pdf"},"version_id":"bd4a6f13-7910-43df-a8c4-f3923a6d6f7b"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Pseudomonas pseudoflava","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"SDS-PAGE","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"P(3HB-co-3HV)","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"PHA synthase","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Total organic carbon","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Polyhydroxyalkanoates (PHA) production from synthetic waste using Pseudomonas pseudoflava : PHA synthase enzyme activity analysis from P. pseudoflava and P. palleronii","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Polyhydroxyalkanoates (PHA) production from synthetic waste using Pseudomonas pseudoflava : PHA synthase enzyme activity analysis from P. pseudoflava and P. palleronii","subitem_title_language":"en"}]},"item_type_id":"79","owner":"18","path":["46","312","372","311"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2018-03-14"},"publish_date":"2018-03-14","publish_status":"0","recid":"9641","relation_version_is_last":true,"title":["Polyhydroxyalkanoates (PHA) production from synthetic waste using Pseudomonas pseudoflava : PHA synthase enzyme activity analysis from P. pseudoflava and P. palleronii"],"weko_creator_id":"18","weko_shared_id":-1},"updated":"2024-02-05T07:06:56.156102+00:00"}