{"created":"2023-06-19T10:29:44.974687+00:00","id":9749,"links":{},"metadata":{"_buckets":{"deposit":"6975dcbb-9b4a-4ea0-a92a-e32d59bfa17d"},"_deposit":{"created_by":18,"id":"9749","owners":[18],"pid":{"revision_id":0,"type":"depid","value":"9749"},"status":"published"},"_oai":{"id":"oai:muroran-it.repo.nii.ac.jp:00009749","sets":["41:227"]},"author_link":["55383"],"item_81_date_granted_17":{"attribute_name":"学位授与年月日","attribute_value_mlt":[{"subitem_dategranted":"2018-09-26"}]},"item_81_degree_grantor_10":{"attribute_name":"学位授与機関","attribute_value_mlt":[{"subitem_degreegrantor":[{"subitem_degreegrantor_language":"ja","subitem_degreegrantor_name":"室蘭工業大学"},{"subitem_degreegrantor_language":"en","subitem_degreegrantor_name":"Muroran Institute of Technology"}],"subitem_degreegrantor_identifier":[{"subitem_degreegrantor_identifier_name":"10103","subitem_degreegrantor_identifier_scheme":"kakenhi"}]}]},"item_81_degree_name_11":{"attribute_name":"学位名","attribute_value_mlt":[{"subitem_degreename":"博士（工学）","subitem_degreename_language":"ja"}]},"item_81_description_25":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_81_description_7":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"近年、人間と同様に「匂い」を識別できる「匂いセンサ」が開発されているが、その性能は人をはじめとする生体におよばないのが現状である。著者は「匂いセンサ」を実現するために、生体機能物質を利用したSurface Acoustic Wave (SAW)デバイスにおける弾性表面波の伝播特性を研究した。本論文は、匂い結合タンパク質を設置したSAWデバイスにおける弾性表面波の伝播特性を理論及び実験により検討した結果について述べている。室蘭工業大学の岩佐研究室では両生類のイモリを対象に嗅覚関連タンパク質を研究し、イモリ嗅上皮に特異的に発現するCynops pyrrhogaster lipocalin 遺伝子を発見した。岩佐らはこの遺伝子からできた匂い結合タンパク質をCp-Lip1と呼んでいる。また、室蘭工業大学の福田研究室では匂いセンサを実現するためにSAWデバイスを研究している。本研究では、このCp-Lip1匂い結合タンパク質とSAWデバイスを匂いセンサに用いている。第2章では、弾性表面波の伝播を理論解析し、SAWデバイスにおける弾性表面波の伝播速度の理論式を導出した。室蘭工業大学の福田研究室で開発したSAWデバイスにおいて、SAWの伝播経路上に匂い結合タンパク質を含む液体層を作成した。この液体層では、レーリー波、漏洩レーリー波、縦波が伝播する。この理論解析により、このSAWデバイスの液体層で伝播するSAWの総伝播速度を明らかにした。第3章では、SAWデバイスにおいて、試料溶液を溝に置いた時のSAWの伝播特性の測定について述べた。測定実験では、IDT電極間距離は25.00mm、圧電材料は42Y-45X STカット水晶基板のSAWデバイスを使った。このSAWデバイスの周波数は157.6MHzである。まず、エタノールとBSAタンパク質を試料溶液として使って、導出したSAWの伝播速度の理論式が有効であることを明らかにした。次に、匂い分子を使った実験で使用する溝の適切なサイズを決めるために、二つの異なるサイズの溝を使ってSAWの受信側IDTの感度を調べた。その結果、溝幅0.41mm-溝高さ0.26mmの溝基板は溝幅0.24mm-溝高さ0.25mmの溝基板よりSAWの受信側IDTの感度が良いことを明らかにした。匂い分子を同定する実験では、Cp-Lip1匂い結合タンパク質に対して5種類の単一匂い分子の影響を調べた。実験では、異なる濃度の5種類の単一匂い分子をSAWデバイス上でCp-Lip1に反応させ、SAWの伝播特性の変化を測定した。第4章では、SAWデバイスにおけるこの5種類の単一匂い分子の理論速度を計算し、実験結果との比較を述べた。SAWデバイスにおける弾性表面波の実験結果と理論計算の伝播速度はほぼ一致していた。以上のように、本論文は、Cp-Lip1を用いたSAW型匂いセンサを使って匂い分子を同定できる可能性があることを示した。","subitem_description_language":"ja","subitem_description_type":"Abstract"},{"subitem_description":"Biological function-oriented odor sensors are required to fulfil needs for various applications of biological electronic nose systems. Surface acoustic wave (SAW) devices, in which Rayleigh waves propagate, have also been studied for biological function-oriented odor sensors. I describe measurement of SAWs velocities to identify five odorant molecules by using a SAW device as the biological function odor sensor. Dr. Tatsuo Iwasa in Muroran Institute of Technology has studied olfactory related proteins in amphibian newts. He found a Cynops pyrrhogaster lipocalin (Cp-Lip1) protein, an odorant-binding protein discovered in newts, which can combine with various hydrophobic odorant molecules. Dr. Hisashi Fukuda in Muroran Institute of Technology has studied SAW devices to realize odor sensors. In this study, I used the Cp-Lip1 odorant-binding protein and the SAW device as an odor sensor. In chapter 2, I determined a new mathematical formula for estimating the velocity of SAWs. Surface acoustic waves propagate from InterDigital Transducer (IDT) generating leaky Rayleigh waves when liquid is on the quartz substrates in the SAW device developed in Dr. Fukuda laboratory. The Leaky Rayleigh waves travel radiating longitudinal waves into a liquid layer. On the other hand, Rayleigh waves propagate in the vacuum-solid interface. In chapter 3, I measured the propagation velocities in a SAW device in which ethanol, BSA protein and five kinds of odorant molecules were used as liquid medium. I used ethanol and BSA protein as sample solutions and clarified that the theoretical formula for estimating the velocity of SAWs is effective. In order to determine the appropriate size of the groove two different sizes of grooves were examined. The groove with width-height of 0.41mm-0.26mm showed higher intensity than the groove with width-height of 0.24mm-0.25mm did. Then five kinds of odorant molecule solutions were placed on the SAW device which had the groove with width-height is 0.41mm-0.26mm. I identified five kinds of odorant molecules in the measurement of SAWs velocities. In chapter 4, I compared between the theoretical and the experimental velocities in order to verify validity of the SAW device for the biological function-oriented odor sensor. I showed that the theoretical and the experimental propagation velocities of the five kinds of odorant molecule solutions were nearly identical. I have described that our frequency equation can predict different odorant molecules in the SAW device. My study provides the basis for a new method for developing biological function-oriented odor sensors.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_81_dissertation_number_13":{"attribute_name":"学位授与番号","attribute_value_mlt":[{"subitem_dissertationnumber":"甲第424号"}]},"item_81_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.15118/00009699","subitem_identifier_reg_type":"JaLC"}]},"item_81_subject_9":{"attribute_name":"日本十進分類法","attribute_value_mlt":[{"subitem_subject":"576","subitem_subject_scheme":"NDC"}]},"item_81_text_12":{"attribute_name":"学位の種別","attribute_value_mlt":[{"subitem_text_language":"ja","subitem_text_value":"課程博士"}]},"item_81_text_14":{"attribute_name":"報告番号","attribute_value_mlt":[{"subitem_text_language":"ja","subitem_text_value":"甲第424号"}]},"item_81_text_15":{"attribute_name":"学位記番号","attribute_value_mlt":[{"subitem_text_language":"ja","subitem_text_value":"博甲第424号"}]},"item_81_text_16":{"attribute_name":"研究科・専攻","attribute_value_mlt":[{"subitem_text_language":"ja","subitem_text_value":"工学専攻"}]},"item_81_version_type_24":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorAffiliations":[{"affiliationNameIdentifiers":[],"affiliationNames":[{"affiliationName":""}]}],"creatorNames":[{"creatorName":"楊, 玉霞","creatorNameLang":"ja"},{"creatorName":"YANG, Yuxia","creatorNameLang":"en"}],"familyNames":[{},{}],"givenNames":[{},{}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2018-11-21"}],"displaytype":"detail","filename":"A424.pdf","filesize":[{"value":"4.0 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"A424","objectType":"fulltext","url":"https://muroran-it.repo.nii.ac.jp/record/9749/files/A424.pdf"},"version_id":"8b722463-f81f-4f2d-aa45-c20b3d7b2d48"},{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2018-11-21"}],"displaytype":"detail","filename":"A424_summary.pdf","filesize":[{"value":"279.1 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"A424_summary","objectType":"abstract","url":"https://muroran-it.repo.nii.ac.jp/record/9749/files/A424_summary.pdf"},"version_id":"d5b6e7d9-6038-4d1c-98cb-68c3229be7e4"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"doctoral thesis","resourceuri":"http://purl.org/coar/resource_type/c_db06"}]},"item_title":"生体機能指向型匂いセンサにおける弾性表面波の伝播特性の解析","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"生体機能指向型匂いセンサにおける弾性表面波の伝播特性の解析","subitem_title_language":"ja"},{"subitem_title":"Analysis of surface acoustic wave propagation velocity in biological function-oriented odor sensor","subitem_title_language":"en"}]},"item_type_id":"81","owner":"18","path":["227"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2018-11-21"},"publish_date":"2018-11-21","publish_status":"0","recid":"9749","relation_version_is_last":true,"title":["生体機能指向型匂いセンサにおける弾性表面波の伝播特性の解析"],"weko_creator_id":"18","weko_shared_id":-1},"updated":"2024-01-22T02:40:09.048117+00:00"}