| Item type |
学術雑誌論文 / Journal Article.(1) |
| 公開日 |
2022-03-24 |
| 書誌情報 |
en : BIOENGINEERING-BASEL
巻 8,
号 11,
p. 157,
発行日 2021
|
| タイトル |
|
|
タイトル |
Two-Stage Polyhydroxyalkanoates (PHA) Production from Cheese Whey Using Acetobacter pasteurianus C1 and Bacillus sp. CYR1 |
|
言語 |
en |
| 言語 |
|
|
言語 |
eng |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
cheese whey |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
acetic acid |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Acetobacter pasteurianus C1 |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Bacillus sp. CYR-1 |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
PHA |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
PHB |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| アクセス権 |
|
|
アクセス権 |
open access |
|
アクセス権URI |
http://purl.org/coar/access_right/c_abf2 |
| 著者 |
張, 傛喆
Reddy, Motakatla Venkateswar
Imura, Kazuma
小野寺, 瑠依
Kamada, Natsumi
Sano, Yuki
|
| 室蘭工業大学研究者データベースへのリンク |
|
|
表示名 |
張 傛喆（CHANG Young-Cheol） |
|
URL |
https://rdsoran.muroran-it.ac.jp/html/100000221_ja.html |
| 抄録 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
Cheese whey (CW) can be an excellent carbon source for polyhydroxyalkanoates (PHA)-producing bacteria. Most studies have used CW, which contains high amounts of lactose, however, there are no reports using raw CW, which has a relatively low amount of lactose. Therefore, in the present study, PHA production was evaluated in a two-stage process using the CW that contains low amounts of lactose. In first stage, the carbon source existing in CW was converted into acetic acid using the bacteria, Acetobacter pasteurianus C1, which was isolated from food waste. In the second stage, acetic acid produced in the first stage was converted into PHA using the bacteria, Bacillus sp. CYR-1. Under the condition of without the pretreatment of CW, acetic acid produced from CW was diluted at different folds and used for the production of PHA. Strain CYR-1 incubated with 10-fold diluted CW containing 5.7 g/L of acetic acid showed the higher PHA production (240.6 mg/L), whereas strain CYR-1 incubated with four-fold diluted CW containing 12.3 g/L of acetic acid showed 126 mg/L of PHA. After removing the excess protein present in CW, PHA production was further enhanced by 3.26 times (411 mg/L) at a four-fold dilution containing 11.3 g/L of acetic acid. Based on Fourier transform infrared spectroscopy (FT-IR), and H-1 and C-13 nuclear magnetic resonance (NMR) analyses, it was confirmed that the PHA produced from the two-stage process is poly-beta-hydroxybutyrate (PHB). All bands appearing in the FT-IR spectrum and the chemical shifts of NMR nearly matched with those of standard PHB. Based on these studies, we concluded that a two-stage process using Acetobacter pasteurianus C1 and Bacillus sp. CYR-1 would be applicable for the production of PHB using CW containing a low amount of lactose. |
|
言語 |
en |
| 出版者 |
|
|
出版者 |
MDPI |
|
言語 |
en |
| 出版者版へのリンク |
|
|
表示名 |
10.3390/bioengineering8110157 |
|
URL |
https://doi.org/10.3390/bioengineering8110157 |
| DOI |
|
|
関連タイプ |
isIdenticalTo |
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|
識別子タイプ |
DOI |
|
|
関連識別子 |
10.3390/bioengineering8110157 |
| 日本十進分類法 |
|
|
主題Scheme |
NDC |
|
主題 |
464 |
| 権利 |
|
|
言語 |
en |
|
権利情報 |
https://creativecommons.org/licenses/by/4.0/ |
| 著者版フラグ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| フォーマット |
|
|
内容記述タイプ |
Other |
|
内容記述 |
application/pdf |
Bioeng_8_11_157 (5.2 MB)
