好熱菌由来SODの精製および，その活性と構造に関する研究

好熱菌由来SODの精製および，その活性と構造に関する研究利用統計を見る

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アイテムタイプ	学位論文 / Thesis or Dissertation
言語	日本語
著者	丁凌云
著者（別表記）	DING LING YUN
抄録	Reactive oxygen species such as super oxide anion was produced as a by-product from aerobic respiration metabolism. Because superoxide dismutase (SOD) extinguishes the active oxygen or oxide anion radical, it plays a role in DNA and enzymes protecting against oxygen damage. SOD is a universal antioxidant enzyme in a microbe, a higher animal, a plant. The SOD has a Cu/Zn type, a Mn type, and a Fe type by the species that there is in the active center, and the MnSOD exists both in eukaryote and prokaryote. Thermophilic bacteria can grow under high temperature (more than 55 degrees Celsius). This paper has described purification of MnSOD which is derived from thermophilic bacteria C36 strain by gel chromatography under the presence of surfactant SDS. This new method was achieved the high recovery of SOD to be approximately 33%. It would be able to get a SOD with tolerance (SDS tolerance, heat resistance, pH tolerance) to be the industrial usage. And this new isolation method can apply the other thermophilic bacteria, too. MnSOD has dimeric structure. However, the function of the monomer-dimer conversion of the SOD is not clarified. SODs were chemistry cross linked with divalent reagents such as glutaraldehyde to fix a dimer conformation. The activity of cross-linked SOD largely decreased because molecular structure was fixed. The inter-subunit flexibility of the SOD was shown to be important to present SOD activity.
学位授与機関	室蘭工業大学 (Muroran Institute of Technology)
学位名	博士（工学）
学位の種別	課程博士
学位授与番号	10103甲第375号
報告番号	甲第375号
学位記番号	博甲第375号
学位授与年月日	2015-03-23
日本十進分類法	579
著者版フラグ	ETD
フォーマット	application/pdf

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好熱菌由来SODの精製および，その活性と構造に関する研究利用統計を見る

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好熱菌由来SODの精製および，その活性と構造に関する研究 利用統計を見る

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好熱菌由来SODの精製および，その活性と構造に関する研究利用統計を見る