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好熱菌由来SODの精製および，その活性と構造に関する研究
https://doi.org/10.15118/00005131
https://doi.org/10.15118/000051312ba2a41a-b1e6-40ac-b352-f0da0d326720
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A375 (2.1 MB)
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A375_summary (369.7 kB)
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| Item type | 学位論文 / Thesis or Dissertation(1) | |||||||||||
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| 公開日 | 2015-06-11 | |||||||||||
| タイトル | ||||||||||||
| タイトル | 好熱菌由来SODの精製および，その活性と構造に関する研究 | |||||||||||
| 言語 | ja | |||||||||||
| 言語 | ||||||||||||
| 言語 | jpn | |||||||||||
| 資源タイプ | ||||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_db06 | |||||||||||
| 資源タイプ | doctoral thesis | |||||||||||
| ID登録 | ||||||||||||
| ID登録 | 10.15118/00005131 | |||||||||||
| ID登録タイプ | JaLC | |||||||||||
| アクセス権 | ||||||||||||
| アクセス権 | open access | |||||||||||
| アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||||||||
| 著者 |
丁, 凌云
× 丁, 凌云
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| 抄録 | ||||||||||||
| 内容記述タイプ | Abstract | |||||||||||
| 内容記述 | Reactive oxygen species such as super oxide anion was produced as a by-product from aerobic respiration metabolism. Because superoxide dismutase (SOD) extinguishes the active oxygen or oxide anion radical, it plays a role in DNA and enzymes protecting against oxygen damage. SOD is a universal antioxidant enzyme in a microbe, a higher animal, a plant. The SOD has a Cu/Zn type, a Mn type, and a Fe type by the species that there is in the active center, and the MnSOD exists both in eukaryote and prokaryote. Thermophilic bacteria can grow under high temperature (more than 55 degrees Celsius). This paper has described purification of MnSOD which is derived from thermophilic bacteria C36 strain by gel chromatography under the presence of surfactant SDS. This new method was achieved the high recovery of SOD to be approximately 33%. It would be able to get a SOD with tolerance (SDS tolerance, heat resistance, pH tolerance) to be the industrial usage. And this new isolation method can apply the other thermophilic bacteria, too. MnSOD has dimeric structure. However, the function of the monomer-dimer conversion of the SOD is not clarified. SODs were chemistry cross linked with divalent reagents such as glutaraldehyde to fix a dimer conformation. The activity of cross-linked SOD largely decreased because molecular structure was fixed. The inter-subunit flexibility of the SOD was shown to be important to present SOD activity. | |||||||||||
| 言語 | en | |||||||||||
| 学位授与機関 | ||||||||||||
| 学位授与機関識別子Scheme | kakenhi | |||||||||||
| 学位授与機関識別子 | 10103 | |||||||||||
| 言語 | ja | |||||||||||
| 学位授与機関名 | 室蘭工業大学 | |||||||||||
| 言語 | en | |||||||||||
| 学位授与機関名 | Muroran Institute of Technology | |||||||||||
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| 言語 | ja | |||||||||||
| 学位名 | 博士（工学） | |||||||||||
| 学位の種別 | ||||||||||||
| ja | ||||||||||||
| 課程博士 | ||||||||||||
| 学位授与番号 | ||||||||||||
| 学位授与番号 | 甲第375号 | |||||||||||
| 報告番号 | ||||||||||||
| ja | ||||||||||||
| 甲第375号 | ||||||||||||
| 学位記番号 | ||||||||||||
| ja | ||||||||||||
| 博甲第375号 | ||||||||||||
| 学位授与年月日 | ||||||||||||
| 学位授与年月日 | 2015-03-23 | |||||||||||
| 日本十進分類法 | ||||||||||||
| 主題Scheme | NDC | |||||||||||
| 主題 | 579 | |||||||||||
| 著者版フラグ | ||||||||||||
| 出版タイプ | VoR | |||||||||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||||||
| フォーマット | ||||||||||||
| 内容記述タイプ | Other | |||||||||||
| 内容記述 | application/pdf | |||||||||||
