An enzyme immunoassay using antiserum against fatty acid synthetase I(FAS-I; a malony1-CoAdependent，acy1 carrier protein (ACP)-requiring system) from an avirulent mycobacteria，Mycobacterium smegmatis，was examined for detection of cells of this genus. The antiserum was cross-reactive with virulent and avirulent species of mycobacteria such as M.tuberculosis，M.bovis BCG and M.phlei，respectively. Only a weak reactivity was，however，observed with Nocardia sp. possessing both FAS-I and FAS-II(fatty acid synthetase II ; an acety1-CoA-dependent，ACP-requiring system)，and no cross-reactivities were found with bacteria like Eschelichia coli and Bacillus subtilis having only FAS-II. These results suggested that virulent and avirulent Mycobacterium sp. could be detected and identified specifically by the immunoassay method using the enzyme-labeled antiserum aganst FAS-I.
酵素標識免疫法によるヒト型結核菌および近縁菌群の検出
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