| Item type |
学術雑誌論文 / Journal Article.(1) |
| 公開日 |
2022-03-30 |
| 書誌情報 |
en : Water-Energy Nexus
巻 4,
p. 69-75,
発行日 2021
|
| タイトル |
|
|
タイトル |
Validation of biphenyl degradation pathway by polymerase chain reaction, peptide mass fingerprinting and enzyme analysis |
|
言語 |
en |
| 言語 |
|
|
言語 |
eng |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
BphC |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Catechol 2,3-dioxygenase |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Biphenyl degradation |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Peptide mass fingerprinting (PMF) |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Aquamicrobium genus |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| アクセス権 |
|
|
アクセス権 |
open access |
|
アクセス権URI |
http://purl.org/coar/access_right/c_abf2 |
| 著者 |
張, 傛喆
SUGAWARA, Hideto
REDDY, M. Venkateswar
|
| 室蘭工業大学研究者データベースへのリンク |
|
|
表示名 |
張 傛喆（CHANG Young-Cheol） |
|
URL |
http://rdsoran.muroran-it.ac.jp/html/100000221_ja.html |
| 抄録 |
|
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内容記述タイプ |
Abstract |
|
内容記述 |
Our previous studies showed, bacterium Aquamicrobium sp. SK-2 could degrade biphenyl and polychlorinatedbiphenyls (PCBs). In the present study, proteins involved in the biphenyl degradation was evaluatedusing various molecular biology methods. The gene bphC present in the strain SK-2 was identifiedusing the polymerase chain reaction method. Further the key enzyme in biphenyl degradation, 2,3-dihydroxybiphenyl 1,2-dioxygenase (BphC) was purified through anion exchange and gel filtration chromatography,subsequently the enzyme activity was measured. The N-terminal amino acid sequence ofthe purified enzyme showed 92% homology with BphC enzyme of Gram-negative bacteria(Pseudomonas sp. KKS102, Comamonas testosterone, Burkholderiaceae bacterium, Delftia acidovorans, andAchromobacter denitrificans). Fractions collected during protein purification were applied on SDS-PAGEgel. Significant bands were selected in SDS-PAGE gel, and the gel pieces were cut out to analyze the proteinsusing peptide mass fingerprinting (PMF) method. PMF method provided useful information aboutthe proteins involved in biphenyl degradation. Apart from BphC, two other enzymes, benzoate dioxygenaseand catechol 2,3-dioxygenase which were involved in biphenyl degradation process were identified.The results indicate that catechol can be degraded to 2-hydroxymuconic-semialdehyde and this result isin accordance with the results from our previous study. Based on all these results we can conclude thatthe strain SK-2 is a potential candidate for the bioremediation of biphenyl contaminated places._ 2021 The Authors. Production and hosting by Elsevier B.V. on behalf of KeAi Communications Co., Ltd.This is an open access article under the CC BY-NC-ND license |
|
言語 |
en |
| 出版者 |
|
|
出版者 |
Elsevier |
|
言語 |
en |
| 出版者版へのリンク |
|
|
表示名 |
10.1016/j.wen.2021.04.001 |
|
URL |
https://doi.org/10.1016/j.wen.2021.04.001 |
| DOI |
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|
関連タイプ |
isIdenticalTo |
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|
識別子タイプ |
DOI |
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|
関連識別子 |
10.1016/j.wen.2021.04.001 |
| 日本十進分類法 |
|
|
主題Scheme |
NDC |
|
主題 |
437 |
| ISSN |
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|
収録物識別子タイプ |
EISSN |
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収録物識別子 |
2588-9125 |
| 権利 |
|
|
言語 |
en |
|
権利情報 |
https://creativecommons.org/licenses/by/4.0/ |
| 著者版フラグ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| フォーマット |
|
|
内容記述タイプ |
Other |
|
内容記述 |
application/pdf |
WEN_4_69-75 (1.2 MB)
