| Item type |
学術雑誌論文 / Journal Article.(1) |
| 公開日 |
2011-12-20 |
| 書誌情報 |
en : Journal of Industrial Microbiology & Biotechnology
巻 38,
号 10,
p. 1667-1677,
発行日 2011-03-25
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| タイトル |
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タイトル |
Isolation and characterization of tetrachloroethylene- and cis-1,2-dichloroethylene-dechlorinating propionibacteria |
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言語 |
en |
| 言語 |
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|
言語 |
eng |
| キーワード |
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|
言語 |
en |
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主題Scheme |
Other |
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主題 |
Tetrachloroethylene |
| キーワード |
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言語 |
en |
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主題Scheme |
Other |
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主題 |
Cis-1,2-Dichloroethylene |
| キーワード |
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|
言語 |
en |
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主題Scheme |
Other |
|
主題 |
chlorinated aliphatic compounds |
| キーワード |
|
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言語 |
en |
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主題Scheme |
Other |
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主題 |
corrinoid |
| キーワード |
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|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Propionibacteria |
| キーワード |
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|
言語 |
en |
|
主題Scheme |
Other |
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主題 |
PCE dehalogenase |
| 資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
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資源タイプ |
journal article |
| アクセス権 |
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アクセス権 |
open access |
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アクセス権URI |
http://purl.org/coar/access_right/c_abf2 |
| 著者 |
張, 傛喆
IKEUTSU, Kaori
TOYAMA, Tadashi
CHOI, DuBok
菊池, 愼太郎
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| 室蘭工業大学研究者データベースへのリンク |
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張 傛喆（CHANG Young-Cheol） |
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http://rdsoran.muroran-it.ac.jp/html/100000221_ja.html |
| 抄録 |
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内容記述タイプ |
Abstract |
|
内容記述 |
Two rapidly growing propionibacteria that could reductively dechlorinate tetrachloroethylene (PCE) and cis-1,2-dichloroethylene (cis-DCE) to ethylene were isolated from environmental sediments. Metabolic characterization and partial sequence analysis of their 16S rRNA genes showed that the new isolates, designated as strains Propionibacterium sp. HK-1 and Propionibacterium sp. HK-3, did not match any known PCE- or cis-DCE-degrading bacteria. Both strains dechlorinated relatively high concentrations of PCE (0.3 mM) and cis-DCE (0.52 mM) under anaerobic conditions without accumulating toxic intermediates during incubation. Cell-free extracts of both strains catalyzed PCE and cis-DCE dechlorination; degradation was accelerated by the addition of various electron donors. PCE dehalogenase from strain HK-1 was mediated by a corrinoid protein, since the dehalogenase was inactivated by propyl iodide only after reduction by titanium citrate. The amounts of chloride ions (0.094 and 0.103 mM) released after PCE (0.026 mM) and cis-DCE (0.05 mM) dehalogenation using the cell-free enzyme extracts of both strains, HK-1 and HK-3, were stoichiometrically similar (91 and 100%), indicating that PCE and cis-DCE were fully dechlorinated. Radiotracer studies with [1,2-¹⁴C] PCE and [1,2-¹⁴C] cis-DCE indicated that ethylene was the terminal product; partial conversion to ethylene was observed. Various chlorinated aliphatic compounds (PCE, trichloroethylene, cis-DCE, trans-1,2-dichloroethylene, 1,1-dichloroethylene, 1,1-dichloroethane, 1,2-dichloroethane, 1,2-dichloropropane, 1,1,2-trichloroethane, and vinyl chloride) were degraded by cell-free extracts of strain HK-1. |
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言語 |
en |
| 出版者 |
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出版者 |
Springer |
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言語 |
en |
| 出版者版へのリンク |
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10.1007/s10295-011-0956-1 |
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https://doi.org/10.1007/s10295-011-0956-1 |
| DOI |
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関連タイプ |
isVersionOf |
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識別子タイプ |
DOI |
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関連識別子 |
10.1007/s10295-011-0956-1 |
| 論文ID（NAID） |
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関連タイプ |
isVersionOf |
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識別子タイプ |
NAID |
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関連識別子 |
120003674386 |
| 日本十進分類法 |
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主題Scheme |
NDC |
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主題 |
588.51 |
| PMID |
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関連タイプ |
isVersionOf |
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識別子タイプ |
PMID |
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関連識別子 |
21437617 |
| ISSN |
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収録物識別子タイプ |
PISSN |
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収録物識別子 |
13675435 |
| ISSN |
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収録物識別子タイプ |
EISSN |
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収録物識別子 |
14765535 |
| 書誌レコードID |
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収録物識別子タイプ |
NCID |
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収録物識別子 |
AA11132053 |
| 書誌レコードID |
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収録物識別子タイプ |
NCID |
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収録物識別子 |
AA11960951 |
| 権利 |
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言語 |
en |
|
権利情報 |
© Society for Industrial Microbiology 2011 |
| 参考URL |
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識別子タイプ |
URI |
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関連識別子 |
https://www.springerlink.com/content/k4201n1481653348/ |
| 著者版フラグ |
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出版タイプ |
AM |
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出版タイプResource |
http://purl.org/coar/version/c_ab4af688f83e57aa |
| フォーマット |
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内容記述タイプ |
Other |
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内容記述 |
application/pdf |
Chang2.pdf (632.9 kB)
