| Item type |
学術雑誌論文 / Journal Article.(1) |
| 公開日 |
2024-02-05 |
| 書誌情報 |
en : The Journal of Physical Chemistry A
巻 127,
号 6,
p. 1436-1444,
ページ数 9,
発行日 2023-02-05
|
| タイトル |
|
|
タイトル |
Electroabsorption and Stark Fluorescence Spectroscopies of Thioflavin T |
|
言語 |
en |
| 言語 |
|
|
言語 |
eng |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| アクセス権 |
|
|
アクセス権 |
open access |
|
アクセス権URI |
http://purl.org/coar/access_right/c_abf2 |
| 著者 |
Islam, Ahatashamul
Kikuchi, Yudai
飯森, 俊文
|
| 抄録 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
Thioflavin T (ThT) is a typical fluorescent marker for detecting the formation of amyloid fibrils, because its fluorescence intensity increases by more than two orders of magnitude upon complexation with the fibrils. Strong electrostatic fields on protein surfaces are known to be a significant factor in chemical reactions and biological functions. Therefore, ThT bound to amyloid fibrils must experience strong electric fields. This study employed electroabsorption and Stark fluorescence spectroscopies to clarify the effects of external electric fields on the photophysics of ThT. The absorption spectrum shows two bands ascribed to locally excited (LE) and charge transfer (CT) states. Coupling between the LE and CT states is enhanced in the presence of an external electric field, resulting in fluorescence quenching. The electric field strength of the amyloid fibril surface was inferred from the fluorescence quenching efficiency of ThT. |
|
言語 |
en |
| 出版者 |
|
|
出版者 |
American Chemical Society |
|
言語 |
en |
| 出版者版へのリンク |
|
|
URL |
https://doi.org/10.1021/acs.jpca.2c07794 |
| DOI |
|
|
関連タイプ |
isVersionOf |
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
10.1021/acs.jpca.2c07794 |
| ISSN |
|
|
収録物識別子タイプ |
PISSN |
|
収録物識別子 |
1089-5639 |
| 権利 |
|
|
言語 |
en |
|
権利情報 |
© 2023 American Chemical Society |
| 著者版フラグ |
|
|
出版タイプ |
AM |
|
出版タイプResource |
http://purl.org/coar/version/c_ab4af688f83e57aa |
Manuscript_thioflavinT-R1-3-A.pdf (943 KB)
