| Item type |
学術雑誌論文 / Journal Article.(1) |
| 公開日 |
2020-07-09 |
| 書誌情報 |
en : International Journal of Molecular Sciences
巻 21,
号 6,
発行日 2020
|
| タイトル |
|
|
タイトル |
Real-Time 3D Imaging and Inhibition Analysis of Various Amyloid Aggregations Using Quantum Dots |
|
言語 |
en |
| 言語 |
|
|
言語 |
eng |
| キーワード |
|
|
言語 |
ja |
|
主題Scheme |
Other |
|
主題 |
amyloidosis; amyloid β |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
tau |
| キーワード |
|
|
言語 |
ja |
|
主題Scheme |
Other |
|
主題 |
α-synuclein |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
amyloid aggregation inhibitor |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
quantum dot |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
4D-imaging |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| アクセス権 |
|
|
アクセス権 |
open access |
|
アクセス権URI |
http://purl.org/coar/access_right/c_abf2 |
| 著者 |
リン, シュウグアン
GALAQIN, Nuomin
TAINAKA, Reina
島森, 圭弥
倉賀野, 正弘
NOGUCHI, Taro. Q. P
徳樂, 清孝
|
| 室蘭工業大学研究者データベースへのリンク |
|
|
表示名 |
徳樂 清孝（TOKURAKU Kiyotaka） |
|
URL |
http://rdsoran.muroran-it.ac.jp/html/100000137_ja.html |
| 抄録 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
Amyloidosis refers to aggregates of protein that accumulate and are deposited as amyloid fibrils into plaques. When these are detected in organs, they are the main hallmark of Alzheimer’s disease, Parkinson’s disease, and other related diseases. Recent medical advances have shown that many precursors and proteins can induce amyloidosis even though the mechanism of amyloid aggregation and the relationship of these proteins to amyloidosis remains mostly unclear. In this study, we report the real-time 3D-imaging and inhibition analysis of amyloid B (AB ), tau, and a-synuclein aggregation utilizing the a nity between quantum dots (QD) and amyloid aggregates. We successfully visualized these amyloid aggregations in real-time using fluorescence microscopy and confocal microscopy simply by adding commercially available QD. The observation by transmission electron microscopy (TEM) showed that QD particles bound to all amyloid fibrils. The 3D-imaging with QD revealed di erences between amyloid aggregates composed of di erent amyloid peptides that could not be detected by TEM.We were also able to quantify the inhibition activities of these proteins by rosmarinic acid, which has high activity for AB aggregation, from fluorescence micrographs as half-maximal e ective concentrations. These imaging techniques with QD serve as quick, easy, and powerful tools to understand amyloidosis and to discover drugs for therapies. |
|
言語 |
en |
| 出版者 |
|
|
出版者 |
MDPI |
|
言語 |
en |
| 出版者版へのリンク |
|
|
表示名 |
10.3390/ijms21061978 |
|
URL |
https://doi.org/10.3390/ijms21061978 |
| DOI |
|
|
関連タイプ |
isIdenticalTo |
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
10.3390/ijms21061978 |
| 日本十進分類法 |
|
|
主題Scheme |
NDC |
|
主題 |
499 |
| 権利 |
|
|
言語 |
en |
|
権利情報 |
©2020 by the authors; licensee MDPI AG, Basel, Switzerland. This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution License(http://creativecommons.org/licenses/by/4.0/) |
| 著者版フラグ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| フォーマット |
|
|
内容記述タイプ |
Other |
|
内容記述 |
application/pdf |
IJMS_2020_21_1978 (2.1 MB)
